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Avian chlamydiosis is a infection that is bacterial by Chlamydia bacteria. The tests that are currently available Chlamydia species centered on hereditary product. Scientists from Wageningen Bioveterinary Analysis (WBVR) created a test centered on antibodies present in animal blood that will figure out which Chlamydia types infected the animal.
Chlamydia micro-organisms tend to be gram-negative, intracellular micro-organisms. The microbial genus presently comprises of fourteen types, each having its very own host that is preferred. A number of these species can not only cause disease in their hosts that are preferred but in addition have actually zoonotic potential. The infamous zoonotic species Chlamydia psittaci was present in a huge selection of bird types, including chicken. This bacterium could cause a form that is severe of in humans, known as parrot fever.
Differentiate between species
C. gallinacea and C. psittaci can infect birds, but both species differ in epidemiology and potential that is zoonotic. It is crucial to own tools that are diagnostic enable detection and distinction between the bacteria species in poultry. The tests that are available predicated on molecular practices (DNA). These examinations just supply information regarding the infection that is current of animals, and work if there are enough bacteria in the samples taken.
A team of WBVR researchers led by Marloes Heijne and Fimme Jan van der Wal*, investigated whether it is possible to develop a test to detect antibodies against Chlamydia species in poultry. “Serology is suitable for monitoring flocks, as antibodies remain present for a time that is long contamination,” describes Heijne. “At present, no differentiating test is present for Chlamydia serology.”
New test
To enable serology that is differentiating a suspension array was designed, with peptides as antigens. These peptides are derived from known immunoreactive Chlamydia proteins. For the majority of these peptides, species-specific seroreactivity in mammalian sera has been described in the literature. The peptides were immobilised on different coloured spheres, a mix of which was added to serum to capture the species-specific antibodies for the suspension array. “In reality, a suspension that is serological is similar to an ELISA. However, using the array one can perform ELISA’s that are multiple one test simultaneously, in one single response. A Luminex”, Heijne explains for read-out of the assay we use dedicated equipment. The test was made suitable for use in several animal species with a number of additional measures. “In our research, we revealed that lots of peptides, which were utilized in the literary works as antigens for Chlamydia serology in animals, also function in detecting and distinguishing antibodies from the two for chicken crucial Chlamydia species.”
Conclusions
The suspension system range can determine antibodies of four various Chlamydia types in mouse sera. The test that is same also able to distinguish between antibodies of C. psittaci and C. gallinacea in chicken sera. Because the reactivity of chicken sera was not the same as that of mice, signals from chicken sera from the field were difficult to interpret. “Well-characterised chicken sera for evaluating the seroreactivity of the peptides are scarce. Nevertheless, results with field sera are consistent with published data on the occurrence of C. gallinacea in Dutch hens that are laying. Using this, we now have shown the evidence of idea of distinguishing serology for antibodies against Chlamydia in chicken making use of peptides in a suspension variety.”
* WBVR specialist Fimme Jan van der Wal is very first composer of the publication that is scientific posted in Veterinary study (2023)54:31
Source: Wageningen University & Research
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